PHOSPHATASE ACTIVITY IN TETRAHYMENA
نویسندگان
چکیده
منابع مشابه
Acid Phosphatase Activity Associated with Phagotrophy in the Ciliate, Tetrahymena
When living in nature Tetrahymena pyriformis is highly phagotrophic. The cell cytoplasm contains food vacuoles formed by the engulfment ot large molecular weight materials, including particulate matter, through the well developed buccal apparatus. However, no food vacuoles are formed when the organism is cultured axenically in chemically defined media containing only low molecular weight consti...
متن کاملActivity of Ribonuclease, Acid Phosphatase, and Phosphodiesterase in Tetrahymena Pyriformis during Growth
Studies on the nucleolytic activity of cell homogenates of Tetrahymena pyriformis indicated that enzyme activity was, among other factors, a function of the age of the culture (1, 2). It was shown that the specific activities of ribonuclease (RNase) (EC 2.7.7.h) and acid phosphatase (APase) (EC 3.1.3.2) in crude homogenates prepared from stationary-phase cultures were consistently higher than t...
متن کاملEffect of different toxic compounds on ATP content and acid phosphatase activity in axenic cultures of Tetrahymena pyriformis.
The sensitivity of protozoa, and particularly ciliated protozoa, to environmental changes suggested a study on the physiological responses arising from exposure to toxic compounds. Tetrahymena pyriformis was used as a test organism in a set of miniaturized assays. The physiological response of this ciliate was assessed in terms of adenosine-5'-triphosphate content and acid phosphatase activity ...
متن کاملImaging Tetrahymena ribozyme splicing activity in single live mammalian cells.
Tetrahymena ribozymes hold promise for repairing genetic disorders but are largely limited by their modest splicing efficiency and low production of final therapeutic proteins. Ribozyme evolution in intact living mammalian cells would greatly facilitate the discovery of new ribozyme variants with high in vivo activity, but no such strategies have been reported. Here we present a study using a n...
متن کاملAssessment of Alkaline Phosphatase Activity in Hydatid Cyst Protoscolices and Liver Tissue as a Pathological Biomarker
Introduction: Hydatid cyst disease is caused by the protoscolices of Echinococcus granulosus. Alkaline phosphatase (ALP) enzyme is required for metabolism, physiology, immunology, and nutrients absorption in parasite. The aim of this study was to compare the level of ALP activity (as a pathological biomarker) in hydatid cyst protoscolices (HCP) with that of sheep liver tissue and to determine t...
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ژورنال
عنوان ژورنال: The Biological Bulletin
سال: 1951
ISSN: 0006-3185,1939-8697
DOI: 10.2307/1538526